Area
Nervous SystemIndication
LeukodystrophiesSystem
In vitroModel references
J Physiol. 2017 Nov 15;595(22):6993-7008;
Human Molecular Genetics, 2017, Vol. 26, No. 13;
J Physiol. 2015 Sep, 15; 593(18):4165-80
Primary quiescent astrocytes (rat, mouse) and human stablished cell lines.
Applications
- Site-directed mutagenesis.
- TALEN nucleases.
- CRISPR.
- RNAi, Adenoviruses and Lentiviruses.
- Transduction.
- Electrophysiology tests (patch-clamp).
- Biochemical tests: image techniques to study cell-cell junction and subcellular localization.
- Biotinilation.
- In vitro transcription.
- Membrane protein biochemistry.
- Protein-protein interactions.
- SPLIT-TEV method.
