Area
GastrointestinalIndication
Nonalcoholic Steatohepatitis (NASH) and Nonalcoholic Fatty Liver disease (NAFLD)System
In vivoModel references
Biochim Biophys Acta. 2015 May;1852(5):1049-58;
Metabolism. 2018 Aug;85: 59-75;
Diabetes. 2016 Oct; 65 (10):3185-99
NAFLD and NASH mouse models. High fat diet and high fructose diet in: male PPAR ß/ơ-KO mice and their wild-type littermates PPAR ß/ơ, male ATF3 -/- and ATF3+/+ mice, male GA- DD45a -/- and ATF3+/+ mice model
Applications
- Validation of molecular markers of hepatic disorders and inflammation.
- Screeening of potential therapeutic compounds for liver diseases.
- Fatty Acid Oxidation Assay.
- Oil red O Staining.
- Cell viability studies.
- EMSA.
- Biochemical and biomolecular tests: gene expres- sion (RT-PCR, qPCR), nuclear fractionation, protein expression and protein phosphorylation (WB).
