System
Ex vivoModel references
Diabetes. 2016 Oct; 65 (10):3185-99
Mouse C2C12 myoblasts- myotubes
Applications
- Gene silencing (siRNA).
- Gene expression (RT-PCR).
- Protein levels (WB).
- EMSA: transcription factors assessment.
- Fatty Acid Oxidation Assay.
- Lipid accumulation in hepatocytes (Oil Red O Staining).
- Deoxy-D-glucose,2-[1,2-3H(N)] Uptake Experiments.