Experimental models

Area

Gastrointestinal

Indication

Nonalcoholic Steatohepatitis (NASH) and Nonalcoholic Fatty Liver disease (NAFLD)

System

In vivo

Model references

Biochim Biophys Acta. 2015 May;1852(5):1049-58;
Metabolism. 2018 Aug;85: 59-75;
Diabetes. 2016 Oct; 65 (10):3185-99

NAFLD and NASH mouse models. High fat diet and high fructose diet in: male PPAR ß/ơ-KO mice and their wild-type littermates PPAR ß/ơ, male ATF3 -/- and ATF3+/+ mice, male GA- DD45a -/- and ATF3+/+ mice model

Applications

  • Validation of molecular markers of hepatic disorders and inflammation.
  • Screeening of potential therapeutic compounds for liver diseases.
  • Fatty Acid Oxidation Assay.
  • Oil red O Staining.
  • Cell viability studies.
  • EMSA.
  • Biochemical and biomolecular tests: gene expres- sion (RT-PCR, qPCR), nuclear fractionation, protein expression and protein phosphorylation (WB).

Principal investigators

Manuel Vázquez-Carrera.

Other group publications

PubMed publications.

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