A recombinant gluten degrading enzyme with an application as an oral supplement to prevent side effects of gluten intake in celiac disease patients or other gluten-mediated conditions.<\/p>\n
Celiac disease is a chronic autoimmune disorder that affects individuals with sensitization to dietary gluten. It is caused by partially degraded gluten peptides, which are immunogenic. These peptides resist further cleavage by digestive peptidases, and they cross the mucosal epithelium of the small intestine and trigger a severe pro-inflammatory autoimmune response. Currently, there is no treatment and patients must adhere to a lifelong strict gluten-free diet. However, gluten-free diets do not provide balanced nutrition, and many celiac suffer intestinal symptoms even with adherence to such dietary restrictions. Overall, this has created a demand for an effective therapy.<\/p>\n
The recombinant enzyme, that cleaves the immunogenic gluten peptides, can be used for oral enzyme therapy. It was assayed with a mouse model for celiac disease and in a dynamic gastrointestinal simulator, which mimics the human gut. In all series of assays, the enzyme, acting at gastric level drastically reduced the number of toxic peptides at low enzyme-to-substrate molar ratios, before reaching the intestine. Also, it was assayed in comparison to another endopeptidase that shows glutenase activity, and it performed significantly better in gliadin degradation assays.<\/p>\n
In vivo proof of concept in a celiac disease mice model has been already performed with satisfactory results.<\/p>\n","protected":false},"excerpt":{"rendered":"
Executive summary A recombinant gluten degrading enzyme with an application as an oral supplement to prevent side effects of gluten…<\/p>\n","protected":false},"author":2,"featured_media":22917,"parent":1398,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-tecnologies.php","meta":{"_acf_changed":false,"inline_featured_image":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"class_list":["post-22578","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/pages\/22578","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/comments?post=22578"}],"version-history":[{"count":5,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/pages\/22578\/revisions"}],"predecessor-version":[{"id":24546,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/pages\/22578\/revisions\/24546"}],"up":[{"embeddable":true,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/pages\/1398"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/media\/22917"}],"wp:attachment":[{"href":"https:\/\/www.fbg.ub.edu\/en\/wp-json\/wp\/v2\/media?parent=22578"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}