Tecnologies per llicenciar

A new approach to combat antibiotic multiresistance in Salmonella and other enteric bacteria (i.e Klebsiella, E. coli)

Advantages

  • This approach opens a new perspective in the prevention and treatment of some infections caused by antibiotic multiresistant enterobacteria.
  • At farm level it can reduce the prevalence of infections caused by several enterobacteria, such as Salmonella in pigs and poultry.

Goal for early future

Ongoing vaccination experiments with mice to show that the identified antigen can protect against infections by AMR Salmonellae.

Intellectual Property

WO2019077109

Reference

UBTT0320

Contact

Inma Íñiguez
Email: iiniguez@fbg.ub.edu
Tel: +34 934 039 798

Executive summary

A molecular microbiology research group has shown that a group of resistance plasmids (IncHI plasmids) that play a relevant role in antibiotic resistance dissemination in enteric bacteria (Salmonella, E. coli, Klebsiella, etc.), encode an interesting extracellular protein. This large molecular mass protein could be useful for the development of therapies for the treatment and/or prophylaxis of bacterial infections where drug resistance encoded by these plasmids has been detected.

The group is looking for a license, but other collaborations may be considered.

Introduction

Although antibiotic treatments have been improved in the last years, pathogenic bacteria have also evolved to overcome those treatments by acquiring multiple antibiotic resistance (AMR) determinants. Many of them are transmitted by plasmids.

AMR determinants encoded by IncHI plasmids are widespread in the Enterobacteriaceae family. Within the genus Salmonella, they account for a significant proportion of AMR phenotypes in the most common invasive Salmonella serovars: S. enterica serovar Typhi and Paratyphi.

Salmonella is a major pathogen in humans as well as in animals. S. enterica  Typhi causes typhoid fever in humans, whereas other non-typhoid Salmonella serotypes can cause a wide spectrum of diseases in humans and animals, such as acute gastroenteritis, bacteriemia, and extraintestinal infections involving many organs.  Other AMR enterobacteria such as E. coli and Klebsiella strains are also the aethiologic agents of severe infections.

Moreover, in a recent publicationin the field, a fragment of IncHI plasmid in E.Coli carrying resistance to multiple antibiotics has been elucidated. These findings highlight the increasing difficulty in the treatment of infections concerning virulent bacteria lineages and pose a significant threat to global human health.

Due to the need to find new alternatives to combat AMR, the research group has been working on elucidating the function of a large molecular mass protein encoded by gene R0009 of the R27 plasmid (a type of InchHI plasmid).

1mSphere. 2018 Sep-Oct; 3(5): e00486-18

Description

The protein encoded by gene R0009 (RSP) has been detected in the secretome and membrane fractions of S. Typhimurium. Inhibition of the expression of the gene blocks the transfer of the antibiotic resistance determinant. Either the native protein or immunogenic fragments could be useful as antigens to immunize and protect against AMR infections caused by bacteria harbouring IncHI plasmids. As well, antibodies directed against the protein could be used for immunotherapy.

Current stage of development

The research group has characterized the protein and its biological function and location. A protein fragment to be used as antigen has been purified and polyclonal antibodies against this fragment have been obtained. Pre-clinical studies using mice are currently being conducted.

 

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Tags: Life Sciences, Salut i ciències de la vida