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Simultaneous multiple-analyte measurement in biosensor micro-chamber

Advantages

  • Accurate, predetermined and uniformly distributed amount of liquid multiplexed to biosensor micro-chambers.
  • Enhanced contact of the functional liquid with biosensor electrodes, allowing better antigens accumulation, electrodes washing, and biosensor restoring for subsequent analysis.

Goal

Industrial partners are sought to further develop the technology through a license agreement.

Intellectual Property

International Patent application (Priority date: May 2017).

Reference

UBTT0312

Contact

Eva Martín, PhD
Email: emartin@fbg.ub.edu
Tel: +34 934 031 995

Executive summary

A new method for the simultaneous detection of multiple analytes in a liquid is presented. A set of micro-chambers with a plurality of biosensors within is operated under pulsating flow mode, assuring the same liquid flux per channel. A prototype has been successfully tested for in-situ analysis of pollutants in seawater. Partners are sought to license the patent.

Introduction

On-site biosensor measurements reduce the sample handling and time constraints associated with conventional analytical chemistry. Current multi-analyte biosensor devices are based in micro-fluidic systems in which the liquid is automatically injected into the micro-chamber at constant velocity liquid flow. In this kind of configuration, the distribution of the liquid among the various micro-chambers is determined only by the geometry of the fluidic paths, which need to be highly symmetric in order to assure a same amount of liquid reaching each micro-chamber. However, the main drawback of such schemes relies on the difficulty to supply the biosensor micro-chambers with a sufficiently precise liquid low flow rate, causing different amounts of liquid entering the biosensor micro-chambers.

Description

Researchers from the University of Barcelona, the Sant’Anna School of Advanced Studies, the Spanish National Research Council, and the Biomedical Research Networking Centers have developed a new method to assure the same quantity of flux per channel in multi-analyte biosensor devices. With this measuring approach, the micro-chambers containing the respective biosensors are flooded cyclically, one at a time, with a pulsing liquid flow through sequentially temporized micro-valves. This liquid subdivision (multiplexing) is driven by a microprocessor firmware controlling the opening/closing of the micro-valves according to analytes and biosensors requirements.

Pulsing flow mode, as opposed to continuous flow, exhibits the advantage of alternating turbulent and rest regimes in the micro-chamber allowing a much better efficiency of the functional liquid during typical biosensors operations such as antigens accumulation, electrodes washing and regeneration. Turbulence favours a true contact of the functional liquid with biosensor electrodes, counteracting typical surface effects arising in continuous, constant low-rate flow which almost prevent liquid circulation at biosensor electrodes surface. Rest intervals, in turn, allow good sedimentation in the biosensor electrodes surface.

Current stage of development

A functional 8-channels prototype developed in the framework of the Sea-on-a-chip European project has been successfully tested in aquaculture facilities for the in-situ analysis of 7 pollutants in seawater (including antifoulants, endocrine disruptors, POPs, biotoxins, and pesticides and antibiotics).

 

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Tags: Physical Sciences, Sistemas industriales