Tecnologías para licenciar

NEW ANTARCTIC MARINE BIOPOLYMER

Goal

The group is looking for a license agreement, but other collaborations may be considered.

Reference

AVCRI232

Contact

Inma Iñiguez
Email: iiniguez@fbg.ub.edu
Tel: +34 934 039 798

Jóse Conde
Email: jconde@fbg.ub.edu
Tel: +34 934 020 148

Summary

We present a marine biopolymer (MBP) from a novel Antarctic bacterium (strain ID1). Its new structure and properties makes it a potential new cosmetic active ingredient which provides both elasticity and strong dermo-protection to human skin.

Scientific background

Since 1995 our group has been focused on the isolation of new Antarctic microorganisms that produce biopolymers. The current project is based on the discovery of a new biopolymer produced by a novel Antarctic bacterium (ID1) isolated from marine sediment from South Shetland Islands.

South Shetland Islands are a group of Antarctic islands showing a surprising variety of microclimates including extreme ranges of temperatures, strong winds, high salinity, and scarce nutrients. These extreme environments have forced all life, including microorganisms, to acclimatise by developing protective mechanisms to survive, one of which being the production of biopolymers.
Biopolymers serve many functions for microorganisms such as protection against dryness, extreme temperatures, high salinity and toxic compounds. At the same time, biopolymers promote adhesion and are a reserve of nutrients. We have demonstrated that the new MBP accomplish most of these functions and therefore it could be applied to protect human skin from the impact of aggressive conditions. Harsh conditions for our skin could be those that occur in our own urban habitat like pollution, or those that arise from activities such as intense sport, sudden temperature changes or loss of nutrients and elasticity due to aging.

IN VIVO CLINICAL EFFICACY

1. MBP increases skin elasticity

Efficacy tests were made on volunteers from 24 to 64 years old. In only 9 days, volunteers treated with a cream containing 0.2% of MBP showed a significant increase (*) in skin elasticity compared with those treated with the same cream without MBP (net-elasticity measured with a Cutometer®) (Fig. 1).

2. MBP shows dermo-protective activity

Volunteers treated with a cream containing 1% of MBP during 9 days showed a significantly lower increase of the Transepidermal water loss after application of an irritant agent (TEWL, measured with a Tewameter®, Fig.2). This demonstrates that the MBP is a dermo-protective agent that strengthens the skin against external aggressions.


Fig. 1. Skin elasticity


Fig. 2. Skin water loss

CLINICAL SAFETY STUDIES

The following safety tests were performed with the MBP (2%) for its potential application in cosmetics.
1. Patch test. Skin compatibility after single application under patch showed. Result: good skin compatibility

2. HRIPT. Human Repeated Insult Patch Test. Result: very good skin compatibility and does not induce any allergenic reaction. Therefore, is possible to claim it is a hypoallergenic product.

3. NNR. Irritant potential measured on fibroblasts from rabbit cornea. Result: negligible cytotoxicity.

4. HET-CAM. Irritant potential by application on chorioallantoic membrane of embryonated chicken eggs. Result: Slight irritant cathegory.

5. Strain ID1 is non-pathogenic

6. Strain ID1 has not been genetically modified

IN VITRO ASSAYED PROPERTIES

1. Highly emulsifying.

MBP forms long-term stable emulsions at lower concentrations than other commercial emulsifiers. Together with its efficacy, this property could help in cosmetic formulations (Fig.3).


Fig.3. Emulsifying activity of the MBP (1%) against cosmetic oil (w/o 1:2 v/v)

2. Cryoprotectant

MBP has a cryoprotectant function against the loss of viability due to freezing. Survival rates of strain ID1 protected by the MBP after freezing cells at -80 ºC, expressed as the percentage of viable cells with respect to unfrozen cells, was 93 % while for cells without MBP was only 49%.


Fig.4. Electron Microscopy image. ID1 cells protected by a layer of MBP

3. Osmoprotectant

MPB has an osmoprotectant function against high concentrations of salts. Survival rates of strain ID1 after being in an hypertonic solution of NaCl 15% for 2 h, expressed as the percentage of viable cells with respect to untreated cells, was 51,8 % while for cells without MBP was only 4,7 %.
Other properties remain to be explored.

Current stage of development

MBP production has been optimized to move to pilot plan fermentation. Initial cost estimation could be provided. We have filed a patent with Priority date: 10/02/2014.

 

 

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Tags: Life Sciences, Salud y ciencias de la vida